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Domain · Technical Depth

Explain the difference between CRISPR knockout, base editing, and prime editing — and why a therapeutics team would reach for one over the others.

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Classic CRISPR-Cas9 makes a double-strand break; the cell repairs it by non-homologous end joining, which introduces indels and knocks a gene out.

The full answer: structure, worked example, likely follow-up.

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