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Judgment & ambiguity · Decisions under uncertainty

A single vial in your master cell bank shows a faint, intermittent mycoplasma signal on PCR, but the culture and the ELISA both read clean. Your scale-up run is scheduled to start Monday. What do you decide?

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I hold the run. An intermittent PCR signal on a master cell bank isn't noise I get to wave away — the cost of being wrong is a contaminated bank feeding every downstream lot, and t

The full answer: structure, worked example, likely follow-up.

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